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HOTDROPS: Ultrahigh-throughput platform for the screening of thermostable proteins


Ultrahigh-throughput platform for the screening of thermostable proteins by thermophilic in vitro transcription-translation and microfluidics

GalChimia, Novozyme, Biochemize, Drop-Tech and four Academic groups participate in the research project HotDrops, whose goal is to develop a new ultrahigh-throughput platform to search for new biocatalysts, with which large enzyme-variant libraries that were previously challenging to handle can be screened. Single bacterial cells or in-vitro transcription/translation systems can be compartmentalized into droplets, each expressing a different gene library variant. This project combines three sciences: enzymology, microfluid technology, and organic chemistry. The project has been funded by the European Union through the Seventh Framework Programme (FP7).

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Comunication and Dissemination

  • Article Biochemical and structural characterization of a novel thermophilic esterase EstD11 provide catalytic insights for the HSL family, published  in Computational and Structural Biotechnology Journal, 2021, 19(7), 1214–1232. DOI: 10.1016/j.csbj.2021.01.047 (Open Access)
  • Article Reactivity of a Recombinant Esterase from Thermus thermophilus HB27 in Aqueous and Organic Media, published in Microorganisms 2022,10, 915. DOI: 10.3390/microorganisms10050915 (Open Access)
  • Poster Design and synthesis of a library of chromogenic chemical entities for the discovery of new thermostable enzymes relevant for the chemical industry presented at EFMC-ISMC (Ljubljana, 2018).


GalChimia thanks all the partners in the HotDrops project for their involvement and teamwork, which have been key to the success of the project.

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